PXD008833 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Zc3h10 is a novel mitochondrial regulator |
Description | Mitochondria are the energy-generating hubs of the cell. In spite of considerable advances, our understanding of the factors that regulate the molecular circuits that govern mitochondrial function remains incomplete. Using a genome-wide functional screen, we have identified the poorly characterized protein Zinc finger CCCH-type containing 10 (Zc3h10) as regulator of mitochondrial physiology. We show that Zc3h10 is upregulated during physiological mitochondriogenesis such as myoblasts differentiation into myotubes. Zc3h10 overexpression boosts mitochondrial function and promotes myoblasts differentiation. On the other hand, depletion of Zc3h10 results in impaired myoblasts differentiation, mitochondrial dysfunction, reduced expression of electron transport chain (ETC) subunits and blunted TCA cycle flux. Notably, we have identified a loss-of-function mutation of Zc3h10 in humans (Tyr105 to Cys105) that is associated with increased body mass index, fat mass, fasting glucose and triglycerides. Isolated peripheral blood mononuclear cells from Cys105 homozygotes display reduced oxygen consumption rate, some ETC subunit expression and decreased levels of some TCA cycle metabolites that derive in mitochondrial dysfunction. Finally, our study identifies Zc3h10 as a novel mitochondrial regulator. |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-06 |
AnnouncementXML | Submission_2018-03-12_02:29:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | alessandro cuomo |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-02-01 09:08:28 | ID requested | |
1 | 2018-03-06 06:01:24 | announced | |
⏵ 2 | 2018-03-12 02:29:41 | announced | Updated publication reference for PubMed record(s): 29507079. |
Publication List
Audano M, Pedretti S, Cermenati G, Brioschi E, Diaferia GR, Ghisletti S, Cuomo A, Bonaldi T, Salerno F, Mora M, Grigore L, Garlaschelli K, Baragetti A, Bonacina F, Catapano AL, Norata GD, Crestani M, Caruso D, Saez E, De Fabiani E, Mitro N, Zc3h10 is a novel mitochondrial regulator. EMBO Rep, 19(4):(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Zc3h10, mitochondria, energy metabolism, oxidative phosphorylation. |
Contact List
Nico Mitro |
contact affiliation | DiSFeB, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milano, 20133, Italy, |
contact email | nico.mitro@unimi.it |
lab head | |
alessandro cuomo |
contact affiliation | IEO |
contact email | alessandro.cuomo@ieo.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008833
- Label: PRIDE project
- Name: Zc3h10 is a novel mitochondrial regulator