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PXD008830-2

PXD008830 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRBPome of primary mouse T helper cells
DescriptionPost-transcriptional gene regulation by mRNA-binding proteins (RBPs) ensures an important layer to control protein outcome. It includes many fundamental processes, ranging from splicing to transport, decay and translation. Although recent technical developments allow the identification of mRNA-binding proteins, the total RBPome of different primary immune cells remains unknown. Here, we performed an RBP capture of the two major primary T helper cell types in mouse: T helper effector-like and regulatory-like (Foxp3+) cells. The two datasets show a high overlap of ~80% to reveal a high confidence common T helper cell RBPome of 240 proteins. 60% of those proteins have not been previously associated with any immune function. Most importantly, 11 RBPs were not previously identified through any mammalian mRNA-capture approach. These novel RBPs include some well-known T cell proteins associated with non-immune functions such as Stat1 and Vav1. Overall our data suggest an underestimated complexity of gene regulatory pathways.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:23:31.588.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Wierer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-02-01 01:54:16ID requested
12021-07-01 08:35:24announced
22024-10-22 05:23:31announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: RBP, mRNA interactome capture, immune cells
Contact List
Matthias Mann
contact affiliationDepartment of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany
contact emailmmann@biochem.mpg.de
lab head
Michael Wierer
contact affiliationDepartment of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact emailwierer@biochem.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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