PXD008788 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE) |
Description | In the present study, we developed a novel targeted proteomics approach for quantification of allele-specific protein expression (ASPE) based on scheduled high resolution multiple reaction monitoring (sMRM-HR) with a heavy stable isotope-labeled quantitative concatamer (QconCAT) internal protein standard. This strategy was applied to the determination of the ASPE of UGT2B15 in human livers using the common UGT2B15 nonsynonymous variant rs1902023 (i.e. Y85D) as the marker to differentiate expressions from the two alleles. This novel ASPE approach has the potential to be widely utilized for the identification of cis-genetic variants capable of regulating gene expression at the protein level. |
HostingRepository | PRIDE |
AnnounceDate | 2018-08-28 |
AnnouncementXML | Submission_2018-08-28_07:46:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jian Shi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-29 03:46:14 | ID requested | |
⏵ 1 | 2018-08-28 07:46:34 | announced | |
Publication List
Shi J, Wang X, Zhu H, Jiang H, Wang D, Nesvizhskii A, Zhu HJ, Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Based Proteomics Method. J Proteome Res, 17(10):3606-3612(2018) [pubmed] |
Keyword List
submitter keyword: Human, Liver, Allele-Specific Protein Expression |
Contact List
Hao-Jie Zhu, Ph.D. |
contact affiliation | Department of Clinical Pharmacy, University of Michigan College of Pharmacy |
contact email | hjzhu@med.umich.edu |
lab head | |
Jian Shi |
contact affiliation | University of Michigan |
contact email | jianshi@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008788
- Label: PRIDE project
- Name: A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)