PXD008765 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Localised protein expression changes in different regions of human Alzheimer’s brain correlate with disease severity - Entorhinal Cortex |
Description | Human brain tissue was obtained from the New Zealand Brain Bank from donors with Alzheimer's disease (AD) or age-matched controls (n=9/group). Mean post mortem delays were ~12h. Six distinct brain regions were dissected, Hippocampus, Cingulate Gyrus, Entorhinal Cortex (severely affected), Motor and Sensory Cortex (less affected) and Cerebellum (spared). Protein was extracted from each sample and, each region was analysed independently. For each region, samples were divided into 3 sets of 3 cases and 3 controls and assigned to an iTRAQ 8 plex, alongside two aliquots of a pooled QC sample. Samples were digested, iTRAQ labelled and analysed. For each region, between 3000 and 4200 proteins were compared between AD and control, identifying a series of known and novel protein expression changes associated with the progression of AD. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-16 |
AnnouncementXML | Submission_2019-02-15_17:47:37.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Unwin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | QSTAR |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-26 06:56:27 | ID requested | |
⏵ 1 | 2019-02-15 17:47:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, Brain, Alzheimer’s, Dementia, iTRAQ, Entorhinal Cortex |
Contact List
Richard David Unwin |
contact affiliation | Division of Cardiovascular Sciences, School of Medical Science, Faculty of Biology, Medicine and Health, The University of Manchester |
contact email | r.unwin@manchester.ac.uk |
lab head | |
Richard Unwin |
contact affiliation | University of Manchester |
contact email | r.unwin@manchester.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008765 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008765
- Label: PRIDE project
- Name: Localised protein expression changes in different regions of human Alzheimer’s brain correlate with disease severity - Entorhinal Cortex