PXD008760-2

PXD008760 is an original dataset announced via ProteomeXchange.

Title	Exoproteome in a perfusion bioreactor containing antibody-producing CHO cells at various cell densities
Description	Chinese Hamster ovary (CHO) cells are the main platform used to produce recombinant proteins in the biopharmaceutical industry and are commonly cultured in either fed-batch or perfusion mode. However, the optimization of the complex biological systems used in such processes is extremely challenging. Omics approaches can reveal otherwise unknown characteristics of these systems and identify culture parameters that can be manipulated to optimize the cultivation process. Here we have applied proteomic profiling to a monoclonal antibody (mAb) production operated in perfusion mode to explore how cell biology and reactor environment change as the cell density reaches ≥ 200 x 106 cells/mL. The proteomics data show an increase of structural proteins as cell density increase, signs of oxidative stress and changes in glutathione metabolism at very high cell densities. Additionally, metabolomic profiling was carried out. See article “High cell density culture has a maintained exoproteome and metabolome” for more information.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:32.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Lundqvist
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-26 06:30:52	ID requested
1	2018-07-04 05:26:48	announced
⏵ 2	2024-10-22 04:04:40	announced	2024-10-22: Updated project metadata.

Zamani L, Lundqvist M, Zhang Y, Aberg M, Edfors F, Bidkhori G, Lindahl A, Mie A, Mardinoglu A, Field R, Turner R, Rockberg J, Chotteau V, High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome. Biotechnol J, 13(10):e1800036(2018) [pubmed]

10.1002/biot.201800036;

curator keyword: Technical

submitter keyword: Perfusion, Bioreactor, high cell density, monoclonal antibody (mAb),Chinese hamster ovary (CHO), exoproteome, LC-MS/MS

Johan Rockberg
contact affiliation	School of Biotechnology, AlbaNova University Center, Royal Institute of Technol-ogy (KTH), 106 91 Stockholm, Sweden
contact email	johan.rockberg@biotech.kth.se
lab head
Magnus Lundqvist
contact affiliation	KTH (Royal Institute of Technology)
contact email	maglund@kth.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008760
     1. Label: PRIDE project
     2. Name: Exoproteome in a perfusion bioreactor containing antibody-producing CHO cells at various cell densities