PXD008748-1

PXD008748 is an original dataset announced via ProteomeXchange.

Title	Nucleolar-nucleoplasmic shuttling of the RNA-binding macrodomain-containing TARG1 is controlled by DNA damage-induced poly-ADP-ribose and implies functions in ribosome biogenesis
Description	In this project we employed affinity purification mass spectrometry to determine the interactome of the macrodomain containing protein TARG1/OARD1. TARG1 functions as a hydrolase towards mono-ADP-ribosylation (MARylation). TARG1 sowed strong binding to ribosomes and proteins associated with rRNA processing. However, when poly-ADP- ribosylation (PARylation) was present in the cellular lysate, TARG1 was found to interacts with DNA repair and chromatin-associated proteins, including ARTD1, in a PARylation-dependent manner. PARylation events also lead to a change in the subcellular localization of TARG1, which was found to reside in the nucleolus in resting cells, and shuttle to the nucleoplasm upon PARylation
HostingRepository	PRIDE
AnnounceDate	2018-04-23
AnnouncementXML	Submission_2018-04-23_02:31:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Preisinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-24 02:54:53	ID requested
⏵ 1	2018-04-23 02:31:18	announced
2	2018-05-02 23:57:52	announced	Updated publication reference for PubMed record(s): 29712969.
3	2024-10-22 04:04:14	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Human, ADP-ribosylation, AP-MS

Dr Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
lab head
Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008748
     1. Label: PRIDE project
     2. Name: Nucleolar-nucleoplasmic shuttling of the RNA-binding macrodomain-containing TARG1 is controlled by DNA damage-induced poly-ADP-ribose and implies functions in ribosome biogenesis