PXD008741-1

PXD008741 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the cell cycle of procylic form Trypanosoma brucei
Description	We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag technology, we quantified 5,325 proteins across the cell cycle in this parasite, providing a useful resource for the scientific community. Of these, 384 proteins were classified as cell cycle regulated and these were subdivided into nine distinct clusters of temporal regulation. A number of known cell cycle regulators in trypanosomes were detected in these groups, validating our approach, as well as forty novel and essential cell cycle regulated proteins that could be considered as future drug targets. Through cross-comparison to the TrypTag microscopy database, we were able to validate the cell cycle regulated patterns of expression for many of these proteins of unknown function. A convenient interface to access and interrogate these data is also presented.
HostingRepository	PRIDE
AnnounceDate	2018-03-21
AnnouncementXML	Submission_2018-03-21_01:50:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Crozier
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); N-ethylmaleimide derivatized cysteine; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-24 01:06:03	ID requested
⏵ 1	2018-03-21 01:50:04	announced

Crozier TWM, Tinti M, Wheeler RJ, Ly T, Ferguson MAJ, Lamond AI, . Mol Cell Proteomics, 17(6):1184-1195(2018) [pubmed]

curator keyword: Biological

submitter keyword: Trypanosoma brucei, Procyclic, Cell Cycle, TMT, Proteomics

Angus Lamond
contact affiliation	Gene Regulation & Expression, School of Life Sciences, University of Dundee, UK.
contact email	a.i.lamond@dundee.ac.uk
lab head
Thomas Crozier
contact affiliation	University of Cambridge
contact email	t.monteirocrozier@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008741
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the cell cycle of procylic form Trypanosoma brucei