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PXD008741 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of the cell cycle of procylic form Trypanosoma brucei
DescriptionWe describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag technology, we quantified 5,325 proteins across the cell cycle in this parasite, providing a useful resource for the scientific community. Of these, 384 proteins were classified as cell cycle regulated and these were subdivided into nine distinct clusters of temporal regulation. A number of known cell cycle regulators in trypanosomes were detected in these groups, validating our approach, as well as forty novel and essential cell cycle regulated proteins that could be considered as future drug targets. Through cross-comparison to the TrypTag microscopy database, we were able to validate the cell cycle regulated patterns of expression for many of these proteins of unknown function. A convenient interface to access and interrogate these data is also presented.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Crozier
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); N-ethylmaleimide derivatized cysteine; phosphorylated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-24 01:06:03ID requested
12018-03-21 01:50:04announced
Publication List
Crozier TWM, Tinti M, Wheeler RJ, Ly T, Ferguson MAJ, Lamond AI, . Mol Cell Proteomics, 17(6):1184-1195(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Trypanosoma brucei, Procyclic, Cell Cycle, TMT, Proteomics
Contact List
Angus Lamond
contact affiliationGene Regulation & Expression, School of Life Sciences, University of Dundee, UK.
contact emaila.i.lamond@dundee.ac.uk
lab head
Thomas Crozier
contact affiliationUniversity of Cambridge
contact emailt.monteirocrozier@gmail.com
dataset submitter
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Dataset FTP location
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