PXD008721-1

PXD008721 is an original dataset announced via ProteomeXchange.

Title	Nannochloropsis oceanica cultivations display phenotypic heterogeneity in lipid metabolism
Description	By combination of FACS sorting based on lipid sensitive Nile Red staining and small cell number proteomics the occurrence of population heterogeneity was analyzed. First population heterogeneity was identified by Nile Red staining and FACS in N. oceanica cells grown in nitrogen replete and nitrogen deplete media. For each condition two subpopulations(+NP3,+NP4,-NP3,-NP4) were detected and three replicates were sorted. Using the gathered proteome data proteins were identified and quantified with the MaxQuant software. Based on these quantification results a two sample t-test was performed to show significant regulation between +NP3 vs. +NP4 or -NP3 vs. -NP4. Goal was to understand the biological reasons for differences in lipid production between subpopulations.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_04:16:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Harst
SpeciesList	scientific name: Nannochloropsis oceanica strain IMET1; NCBI TaxID: 1333499;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-19 03:56:31	ID requested
⏵ 1	2019-02-15 04:16:49	announced
2	2024-10-22 04:04:13	announced	2024-10-22: Updated project metadata.

Chen C, Harst A, You W, Xu J, Ning K, Poetsch A, . Biotechnol Biofuels, 12():21(2019) [pubmed]

curator keyword: Biological

submitter keyword: Population heterogeneity

Nannochloropsis oceanica

lipid metabolism

small cell number proteomics

Dr. Ansgar Poetsch
contact affiliation	School of Biomedical and Healthcare Sciences, Plymouth University, Plymouth PL4 8AA, United Kingdom
contact email	ansgar.poetsch@plymouth.ac.uk
lab head
Andreas Harst
contact affiliation	Ruhr University Bochum
contact email	andreas.harst@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008721

PRIDE project URI

• PRIDE
  1. PXD008721
     1. Label: PRIDE project
     2. Name: Nannochloropsis oceanica cultivations display phenotypic heterogeneity in lipid metabolism