PXD008686 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The MYO6 interactome using BioID |
Description | The intracellular function of myosin motors requires a number of adaptor molecules, which control cargo attachment, but also fine-tune motor activity in time and space. These motor-adaptor-cargo interactions are often weak, transient or highly regulated. To overcome these problems we use a proximity labelling-based proteomics strategy (BioID) to map the interactome of the unique minus end-directed actin motor MYO6. Our analysis identified several distinct MYO6-adaptor modules including two complexes containing RHO GEFs which we screened using further BioID baits (LRCH3, DOCK7, GIPC1 and ARHGEF12). These complexes emphasise the multifunctionality of MYO6 provides the first in vivo interactome of a myosin motor protein, highlighting the power of this approach in uncovering dynamic and functionally diverse myosin motor complexes. |
HostingRepository | PRIDE |
AnnounceDate | 2018-02-27 |
AnnouncementXML | Submission_2018-02-27_04:49:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tom O'Loughlin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-01-16 05:02:03 | ID requested | |
⏵ 1 | 2018-02-27 04:49:43 | announced | |
Publication List
O'Loughlin T, Masters TA, Buss F, The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics. EMBO Rep, 19(4):(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: MYO6, BioID |
Contact List
Dr. Folma Buss |
contact affiliation | Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, UK |
contact email | fb207@cam.ac.uk |
lab head | |
Tom O'Loughlin |
contact affiliation | CIMR |
contact email | to277@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008686
- Label: PRIDE project
- Name: The MYO6 interactome using BioID