PXD008679-2

PXD008679 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics of chemosensory organs of Varroa destructor mite
Description	Aims of the project is the identification of soluble chemosensory proteins in the olfactory organs of the honeybee mite Varroa destructor. Different families of soluble proteins acting as carriers for odorants have been identified in hexapods, while very limited information is available for other terrestrial Arthropoda. In mites and ticks olfactory organs are located on the distal part of the forelegs and on the capitulum appendages. These two body tagmata and the second pair of legs as control, have been dissected out of phoretics and reproductives Varroa destructor females and protein extracts have been analysed through shotgun proteomics. Protein identification and relative quantification (Label-free quantification, LFQ) has been performed using MaxQuant (version 1.5.8.3). Among the proteins more abundant in appendages bearing chemosensory organs, considering as a model hexapoda soluble olfactory proteins, we have searched for small secreted proteins whose structure include a hydrophobic binding pocket.
HostingRepository	PRIDE
AnnounceDate	2018-04-12
AnnouncementXML	Submission_2018-04-25_06:21:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesca Romana Dani
SpeciesList	scientific name: Varroa destructor; NCBI TaxID: 109461;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-15 03:33:16	ID requested
1	2018-04-12 02:14:26	announced
⏵ 2	2018-04-25 06:21:27	announced	Updated publication reference for PubMed record(s): 29653265.
3	2024-10-22 04:07:10	announced	2024-10-22: Updated project metadata.

Iovinella I, McAfee A, Mastrobuoni G, Kempa S, Foster LJ, Pelosi P, Dani FR, Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals. J Proteomics, 181():131-141(2018) [pubmed]

submitter keyword: LC-MS/MS, olfaction, chemosensory proteins, forelegs, capitulum.

Francesca Romana Dani
contact affiliation	Biology Department, University of Firenze. Italy.
contact email	francescaromana.dani@unifi.it
lab head
Francesca Romana Dani
contact affiliation	University of Firenze
contact email	francescaromana.dani@unifi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008679

PRIDE project URI

• PRIDE
  1. PXD008679
     1. Label: PRIDE project
     2. Name: Shotgun proteomics of chemosensory organs of Varroa destructor mite