PXD008665-2

PXD008665 is an original dataset announced via ProteomeXchange.

Title	The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells
Description	Intercellular communication via extracellular vesicles (EVs) is a highly dynamic and specific process. The interaction between EVs and their target cells, especially immune cells, results in functional and phenotype changes that consequently may play a significant role in various physiological states and the pathogenesis of immune-mediated disorders. Monocytes are the most prominent environment sensing immune cells in circulation, skilled to shape their microenvironments via cytokine secretion and further differentiation. Both the circulating monocyte subset distribution and the blood plasma EV pattern are characteristic for preeclampsia, a pregnancy induced immune-mediated hypertensive disorder. We hypothesized that preeclampsia-associated EVs (PE-EVs) induced functional and phenotypic alterations of monocytes. First we proved EV binding and uptake by THP-1 cells. Cellular origin and protein cargo of circulating PE-EVs were characterized by flow cytometry and mass spectrometry. An altered phagocytosis-associated molecular pattern was found on PE-EVs: an elevated CD47 “don’t eat me” signal and decreased exofacial phosphatidylserine “eat-me” signal were found along with decreased uptake of PE-EVs. PE-EVs induced significantly lower chemotaxis and cell motility, but accelerated cell adhesion of THP-1 cells. PE-EVs caused sustained TNF production of THP-1 cells. PE-EVs induced altered monocyte functions suggest that circulating PE-EVs may have a role in the pathogenesis of preeclampsia.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:51.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lilla Turiák
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-12 05:59:08	ID requested
1	2018-04-09 01:28:56	announced
⏵ 2	2024-10-22 04:07:53	announced	2024-10-22: Updated project metadata.

10.1038/s41598-018-23706-7;

Kov, á, cs Á F, L, á, ng O, Turi, á, k L, Á, cs A, K, ő, hidai L, Fekete N, Alasztics B, M, é, sz, á, ros T, Buz, á, s EI, Rig, ó J, P, á, llinger É, The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells. Sci Rep, 8(1):5426(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Bruker Q-TOF, mass spectrometry, human, flow cytometry, preeclampsia, monocyte,extracellular vesicle

László Drahos
contact affiliation	MS Proteomics Group Reserach Centre for Natural Sciences Hungarian Academy of Sciences
contact email	drahos.laszlo@ttk.mta.hu
lab head
Lilla Turiák
contact affiliation	RCNS HAS, MS Proteomics Research Group
contact email	liliat7@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008665
     1. Label: PRIDE project
     2. Name: The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells