PXD008653-1

PXD008653 is an original dataset announced via ProteomeXchange.

Title	Docosahexaenoic and Arachidonic Free Fatty Acids Exert Different Antitumor Activities on Colorectal Cancer Cells
Description	Two PUFA, docosahexaenoic (DHA, 22: 6n3) and arachidonic acids (ARA, 20: 4n6), as well as their derivatives such as eicosanoids, regulate different activities, which include changes in receptor signaling, the composition of rafts, cell metabolism, and membrane structures. These also modify the function of transcription factors and their target genes, a key step essential for the function of FA-activated signaling. This work is focused to determine the antitumor actions of these compounds linked to the regulation of gene transcription on HT-29 colorectal cancer. For this, we performed antiproliferative antitumour assay, LDH breakage test, caspase-3 production, and proteome changes were assessed by SWATH-MS quantitative proteomics followed by pathway analysis in order to find out which molecular mechanisms were being affected. In all cases tested, DHA exercised antitumor actions to a higher extent than ARA, acting mainly by means of down-regulating most of the proteasome system particles, while ARA presented a heavy effect on all the six DNA replication helicase particles but did not affect proteasome. The results indicated that both DHA and ARA stopped colorectal cancer growth and proliferation, thus they represent the ideal candidates as chemopreventive agents.
HostingRepository	PRIDE
AnnounceDate	2022-01-28
AnnouncementXML	Submission_2022-01-28_05:13:28.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignacio Ortea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-11 02:27:44	ID requested
⏵ 1	2022-01-28 05:13:28	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: colorectal cancer

proteomics

PUFA

DHA

ARA

SWATH

Ignacio Ortea
contact affiliation	Proteomics Unit, IMIBIC
contact email	ignacio.ortea@imibic.org
lab head
Ignacio Ortea
contact affiliation	IMIBIC
contact email	iortea@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008653
     1. Label: PRIDE project
     2. Name: Docosahexaenoic and Arachidonic Free Fatty Acids Exert Different Antitumor Activities on Colorectal Cancer Cells