PXD008652-1

PXD008652 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis on the Mechanism of Iron-Compensation Substitute for Manganese Deficiency of Streptococcus pneumoniae
Description	Iron (Fe) and Manganese (Mn) are essential for bacterial survival and virulence as they are involved in catalysis, infection and biofilm formation. The basal metabolism of pathogenic bacteria would be disturbed by the deficiency of metal ions in the host environment. In this study, we found that Streptococcus pneumoniae (S. pneumoniae) can still grow slowly in the medium without manganese ions. Further ICP-MS determination showed that the iron concentration in the bacterium was increased when the manganese content was decreased. The supplement of iron in the manganese deficient culture medium (MDCM) can recovery the bacterial growth. The quantitative proteome using stable-isotope dimethyl labeling was performed to investigate the adaptive growth mechanism of S. pneumoniae in Mn-deficiency condition. Compared with the bacteria cultured in the normal medium, 25 proteins were down-regulated and 54 proteins were up-expressed with more than 1.2-fold alteration (P < 0.05) in S. pneumoniae cultured with MDCM. A large number of depressed proteins are participated in cell energy metabolism, amino acid synthesis process and oxidation products reduction process. The subsequent detection indicated that both intracellar and extracellular hydrogen peroxide levels of in cells were significantly elevated, and the intracellular ATP levels of were evidently decreased in cells cultured in the absence of manganese, meaning that Mn-deficiency can cause multiple metabolic disorders. More importantly, several iron ABC transporters, such as PiuA/PiaA/PitA/SPD_1609, etc, were over-expressed to uptake more iron from the medium. These results suggest that lacking of manganese only disturbed multiple metabolic processes of S. pneumoniae, but also caused the compensatory effect of iron for manganese Mn which is beneficial to the survival of bacteria in extreme environments.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:28:45.413.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kun Cao
SpeciesList	scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153;
ModificationList	N6,N6-dimethyl-L-lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-11 01:10:33	ID requested
⏵ 1	2024-10-07 13:28:46	announced
2	2024-10-22 06:04:26	announced	2024-10-22: Updated project metadata.

Cao K, Lai F, Zhao XL, Wei QX, Miao XY, Ge R, He QY, Sun X, The mechanism of iron-compensation for manganese deficiency of Streptococcus pneumoniae. J Proteomics, 184():62-70(2018) [pubmed]

10.1016/j.jprot.2018.06.004;

curator keyword: Biological

submitter keyword: Manganese Deficiency,Iron-Compensation,Proteomic,Streptococcus pneumoniae

Xuesong Sun
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
contact email	tsunxs@jnu.edu.cn
lab head
Kun Cao
contact affiliation	Jinan university
contact email	hongyibaixue@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008652
     1. Label: PRIDE project
     2. Name: Proteomic Analysis on the Mechanism of Iron-Compensation Substitute for Manganese Deficiency of Streptococcus pneumoniae