PXD008650-3

PXD008650 is an original dataset announced via ProteomeXchange.

Title	mRNA length and 3’UTR structure govern the translation selectivity in RPS14-haploinsufficient erythroblasts
Description	5q- syndrome is a somatic ribosomopathy linked to the monoallelic deletion of the RPS14 gene and characterized by a proeminent erythroid phenotype. The mechanism of anemia involves an impaired differentiation and increased apoptosis of erythroblasts. We have analyzed total cell extracts from UT7 cells with or without a decrease of RPS14 proteins induced by shRNAs. Our data show that GATA1 protein expression is low in line with a defect in the representation of its mRNA at the ribosome. A global analysis of transcripts on polysomes indicates that translation is selective with a decreased representation of the transcripts with a short coding sequence and UTRs and a highly structured 3’UTR, a subset of transcripts that includes GATA1. Our whole proteome analysis confirms that post-transcriptionally downregulated proteins were encoded by transcripts with a short length and structured 3’UTR. We identified a subset of post-translationally downregulated proteins including ribosomal proteins and translation elongation factors encoded by 5’TOP mRNAs that were enriched on the ribosome. Our results indicate that the thermodynamic characteristics of 3’UTR and in a lesser extend 5’UTR and the transcript length are the determinants of translation selectivity under RPS14 haploinsufficiency conditions and that a post-translational regulation of ribosomal proteins accounts for their decreased content in the cell.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-28_22:21:42.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilie-Fleur GAUTIER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-11 01:07:40	ID requested
1	2021-04-28 05:51:51	announced
2	2021-04-28 22:19:14	announced	2021-04-29: Updated project metadata.
⏵ 3	2021-04-28 22:21:43	announced	2021-04-29: Updated project metadata.
4	2024-10-22 05:21:35	announced	2024-10-22: Updated project metadata.

Boussaid I, Le Goff S, Floquet C, Gautier EF, Raimbault A, Viailly PJ, Al Dulaimi D, Burroni B, Dusanter-Fourt I, Hatin I, Mayeux P, Cosson B, Fontenay M, Integrated analyses of translatome and proteome identify the rules of translation selectivity in RPS14-deficient cells. Haematologica, 106(3):746-758(2021) [pubmed]

10.3324/HAEMATOL.2019.239970;

curator keyword: Biological

submitter keyword: Human, Label free, ribosome, UT7, RPS14, UTR, erythropoiesis

Patrick MAYEUX
contact affiliation	Institut Cochin, INSERM U1016, CNRS UMR8104, Paris Descartes University
contact email	patrick.mayeux@inserm.fr
lab head
Emilie-Fleur GAUTIER
contact affiliation	Plateforme Proteomique 3P5, Université Paris Descartes, Inserm U1016, Institut Cochin, Cnrs UMR8104, France
contact email	ef.gautier.upd@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008650
     1. Label: PRIDE project
     2. Name: mRNA length and 3’UTR structure govern the translation selectivity in RPS14-haploinsufficient erythroblasts