PXD008649-1

PXD008649 is an original dataset announced via ProteomeXchange.

Title	Proteome characterization of Paracoccidioides lutzii conidia by using label free proteomics
Description	Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.
HostingRepository	PRIDE
AnnounceDate	2020-09-10
AnnouncementXML	Submission_2020-09-09_22:29:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CLAYTON BORGES
SpeciesList	scientific name: Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis); NCBI TaxID: 502779;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-10 07:22:40	ID requested
⏵ 1	2020-09-09 22:29:50	announced
2	2024-10-22 05:12:12	announced	2024-10-22: Updated project metadata.

Elias Moreira AL, Milhomem Cruz-Leite VR, O'Hara Souza Silva L, Alves Parente AF, Bail, ã, o AM, Maria de Almeida Soares C, Parente-Rocha JA, Ruiz OH, Borges CL, . Fungal Biol, 124(9):766-780(2020) [pubmed]

curator keyword: Biological

submitter keyword: Paracoccidioidomycosis, Conidia, proteome, virulence factors

CLAYTON LUIZ BORGES
contact affiliation	Biochemistry and molecular biology department, Universidade Federal de Goias, Brazil
contact email	clbluiz2@gmail.com
lab head
CLAYTON BORGES
contact affiliation	Biochemistry and molecular biology department, Universidade Federal de Goias, Brazil
contact email	clbluiz2@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008649
     1. Label: PRIDE project
     2. Name: Proteome characterization of Paracoccidioides lutzii conidia by using label free proteomics