PXD008645-2

PXD008645 is an original dataset announced via ProteomeXchange.

Title	HeLa and U2OS long term culture adhesion isolations
Description	Integrin adhesion complexes have been previously isolated and analysed by mass-spectrometry from cells largely spread on matrix proteins over a couple of hours. Here we wished to determine the composition of adhesion complexes from cells cultured for 72 hours under standard cell culture conditions and identify which integrin heterodimers are utilised under these conditions.
HostingRepository	PRIDE
AnnounceDate	2019-06-03
AnnouncementXML	Submission_2019-06-10_08:49:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008645
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Jones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-10 01:50:29	ID requested
1	2019-06-03 06:02:13	announced
⏵ 2	2019-06-10 08:49:09	announced	Updated publication reference for PubMed record(s): 30361699.
3	2024-10-22 04:07:45	announced	2024-10-22: Updated project metadata.

Lock JG, Jones MC, Askari JA, Gong X, Oddone A, Olofsson H, G, ö, ransson S, Lakadamyali M, Humphries MJ, Str, ö, mblad S, Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis. Nat Cell Biol, 20(11):1290-1302(2018) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Human, Cancer Cell, Cell Culture, Adhesion complex, Integrin, proteomics

Martin J Humphries
contact affiliation	Professor of Biochemistry, Faculty of Biology, Medicine & Health, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, UK
contact email	martin.humphries@manchester.ac.uk
lab head
Matthew Jones
contact affiliation	University of Manchester
contact email	matthew.jones-4@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD008645

PRIDE project URI

• PRIDE
  1. PXD008645
     1. Label: PRIDE project
     2. Name: HeLa and U2OS long term culture adhesion isolations