PXD008602-1

PXD008602 is an original dataset announced via ProteomeXchange.

Title	Altered extracellular matrix protein dynamics distinguishes canine ligament-derived stem cells from fibroblasts
Description	Ligaments are prone to injury and degeneration in humans and animals, however the healing potential of ligament is poor and current treatment options lack effectiveness. Stem cell-based therapies hold potential for treatment of ligament injuries. This study aimed to characterise ligament-derived stem cell (LDSC) and ligament fibroblast populations as well as to identify specific niche components which could be used to promote the survival and function of LDSCs. LDSCs and ligament fibroblasts were isolated from canine cranial cruciate ligament and characterised for clonogenicity, multipotency and marker expression. The extracellular matrix (ECM) composition was characterised using a novel metabolic labelling and mass spectrometry approach. Both cell types demonstrated clonogenicity, with significant differences seen in colony size. Stem cell and tenogenic marker expression was increased in LDSCs compared with ligament fibroblasts, although this was only significant for the MSC marker CD73. Both cell types demonstrated multipotency, however this was more marked in LDSCs. A number of collagens, glycoproteins, proteoglycans and ECM regulators were identified in both the LDSC and ligament fibroblast niche using proteomics, with some differences between cell types, including collagens, decorin and fibronectin. Metabolic labelling of cells demonstrated unique turnover profiles for distinct ECM protein groups, with significant differences in new synthesis seen between cell types for a number of proteins, including collagen, decorin and elastin. In conclusion, different cell populations were observed in canine ligament with differences in niche composition and dynamics seen between the two cell types. These differences could be exploited to aid identification of LDSCs and to promote survival and function of LDSCs for potential therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2019-01-28
AnnouncementXML	Submission_2019-01-28_04:03:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008602
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katie Lee
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	Oxidation; Label:13C(6); Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-05 04:04:36	ID requested
⏵ 1	2019-01-28 04:03:27	announced
2	2024-10-22 04:06:10	announced	2024-10-22: Updated project metadata.

Lee KJ, Comerford EJ, Simpson DM, Clegg PD, Canty-Laird EG, Identification and Characterization of Canine Ligament Progenitor Cells and Their Extracellular Matrix Niche. J Proteome Res, 18(3):1328-1339(2019) [pubmed]

curator keyword: Biological

submitter keyword: Ligament, canine, stem cells, metabolic labelling, proteomics, matrix

Dr Elizabeth Canty-Laird
contact affiliation	Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool
contact email	Elizabeth.Laird@liverpool.ac.uk
lab head
Katie Lee
contact affiliation	University of Liverpool
contact email	leekj@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008602
     1. Label: PRIDE project
     2. Name: Altered extracellular matrix protein dynamics distinguishes canine ligament-derived stem cells from fibroblasts