PXD008579-1

PXD008579 is an original dataset announced via ProteomeXchange.

Title	Protein and peptide turnover measurements using pulsed SILAC-TMT approach
Description	We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved investigation of protein stability.
HostingRepository	PRIDE
AnnounceDate	2018-02-09
AnnouncementXML	Submission_2018-02-09_10:45:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Zecha
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-02 06:37:04	ID requested
⏵ 1	2018-02-09 10:45:58	announced
2	2024-10-22 04:13:01	announced	2024-10-22: Updated project metadata.

Zecha J, Meng C, Zolg DP, Samaras P, Wilhelm M, Kuster B, Peptide Level Turnover Measurements Enable the Study of Proteoform Dynamics. Mol Cell Proteomics, 17(5):974-992(2018) [pubmed]

curator keyword: Technical

submitter keyword: protein turnover, pulsed SILAC, TMT, synthesis rates, degradation rates, proteoforms, splice variants, posttranslational cleavage, posttranslational modification

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany
contact email	kuster@tum.de
lab head
Jana Zecha
contact affiliation	TUM
contact email	jana.zecha@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008579

PRIDE project URI

• PRIDE
  1. PXD008579
     1. Label: PRIDE project
     2. Name: Protein and peptide turnover measurements using pulsed SILAC-TMT approach