PXD008444-2

PXD008444 is an original dataset announced via ProteomeXchange.

Title	Histone-like family of nucleoid structuring protein of A. actinomycetemcomitans promotes biofilm formation and alters protein dynamics of other species within multi-species biofilm
Description	Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of the serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, which were accompanied by substantially lower biofilm formation relative to the wild type. In accordance with this observation, the D7S hns mutant exhibited significantly reduced growth within a seven-species biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species in the biofilm regardless co-cultivated with D7S hns or its parental strain. Proteomics analysis of the strains grown in monocultures confirmed the role of H-NS as a repressor of gene expression in A. actinomycetemcomitans. Interestingly, proteomics analysis of the multispecies biofilms indicated that the A. actinomycetemcomitans wildtype and hns mutant imposed different regulatory effects on the pattern of protein expression in the other species, i.e. mainly Streptococcus spp., Fusobacterium nucleatum, and Veillonella dispar. Gene ontology analysis revealed that a large portion of the differentially regulated proteins was related to translational activity. Taken together, our data suggest that, apart from being a negative regulator of gene expression in A. actinomycetemcomitans, H-NS promotes biofilm formation and may be an important factor for survival of this species within a multispecies biofilm.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:35.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Bao
SpeciesList	scientific name: Actinomyces oris; NCBI TaxID: 544580; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Streptococcus oralis; NCBI TaxID: 1303; scientific name: Aggregatibacter aphrophilus; NCBI TaxID: 732; scientific name: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans); NCBI TaxID: 714; scientific name: Veillonella dispar; NCBI TaxID: 39778; scientific name: Streptococcus mutans; NCBI TaxID: 1309; scientific name: Fusobacterium nucleatum; NCBI TaxID: 851;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-14 01:17:08	ID requested
1	2018-06-05 07:17:52	announced
⏵ 2	2024-10-22 04:42:36	announced	2024-10-22: Updated project metadata.

Bao K, Bostanci N, Thurnheer T, Grossmann J, Wolski WE, Thay B, Belibasakis GN, Oscarsson J, H-NS promotes biofilm formation and alters protein dynamics of other species within a polymicrobial oral biofilm. NPJ Biofilms Microbiomes, 4():12(2018) [pubmed]

10.1038/s41522-018-0055-4;

curator keyword: Biological

submitter keyword: multi-species biofilm, HNS, A. actinomycetemcomitans, label-free quantification

Nagihan Bostanci
contact affiliation	Professor, Department of Dental Medicine (DENTMED), OF
contact email	nagihan.bostanci@ki.se
lab head
Kai Bao
contact affiliation	postdoctoral researcher
contact email	kai.bao@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008444
     1. Label: PRIDE project
     2. Name: Histone-like family of nucleoid structuring protein of A. actinomycetemcomitans promotes biofilm formation and alters protein dynamics of other species within multi-species biofilm