PXD008442 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic characterization of the lung extracellular matrix in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis |
Description | Remodeling of the extracellular matrix (ECM) is a common feature in lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF). Here, we applied a sequential tissue extraction strategy to describe disease-specific remodeling of human lung tissue in disease, using end-stages of COPD and IPF. Our strategy was based on quantitative comparison of the disease proteomes, with specific focus on the matrisome, using data-independent acquisition and targeted data analysis (SWATH-MS). Our work provides an in-depth proteomic characterization of human lung tissue during impaired tissue remodeling. In addition, we show important quantitative and qualitative effects of the solubility of matrisome proteins. COPD was characterized by a disease-specific increase in ECM regulators, metalloproteinase inhibitor 3 (TIMP3) and matrix metalloproteinase 28 (MMP-28), whereas for IPF, impairment in cell adhesion proteins, such as collagen VI and laminins, was most prominent. For both diseases, we identified increased levels of proteins involved in the regulation of endopeptidase activity, with several proteins belonging to the serpin family. The established human lung quantitative proteome inventory and the construction of a tissue-specific protein assay library provides a resource for future quantitative proteomic analyses of human lung tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2018-04-05 |
AnnouncementXML | Submission_2018-04-05_03:41:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Emma Åhrman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-14 01:07:20 | ID requested | |
⏵ 1 | 2018-04-05 03:41:58 | announced | |
Publication List
Å, hrman E, Hallgren O, Malmstr, ö, m L, Hedstr, ö, m U, Malmstr, ö, m A, Bjermer L, Zhou XH, Westergren-Thorsson G, Malmstr, ö, m J, Quantitative proteomic characterization of the lung extracellular matrix in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. J Proteomics, 189():23-33(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, Lung, SWATH-MS, COPD, IPF |
Contact List
Johan Malmström |
contact affiliation | Infection medicine proteomics BMC D13 Klinikgatan 28 22241 Lund Sweden |
contact email | johan.malmstrom@med.lu.se |
lab head | |
Emma Åhrman |
contact affiliation | Lund University |
contact email | emma.ahrman@med.lu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008442
- Label: PRIDE project
- Name: Quantitative proteomic characterization of the lung extracellular matrix in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis