PXD008389 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MUC1(insC) affects vesicular transport in renal epithelial cells |
Description | Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogenous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1(insC) lacks the transmembrane domaine, exhibits aberant cellular topology and hence might gain a function during the pathological process. To get insight into potential pathomechanisms we performed differential proteomics of extracellular vesicles shed by renal epithelia into the urine of patients. The study was based on three ADTKD patients and individual controls applying iTRAQ/LC-MS/MS. A total of 727 proteins were identified across all biological and technical replicates. A proportion of 47 proteins (6.5%) were fold-changed species. GO Term Enrichment analysis revealed proteins with significantly changed expression in ADTKD-associated extracellular vesicles as vesicular transport-associated proteins. Among these VTA1 is involved in the multivesicular body (MVB) pathway similar to the charged multivesicular body protein CHMP2B. VTA1 is also claimed to play roles as a cofactor of the AAA ATPases VPS4A and B in the disassembly of ESCRT III. Protein interaction databases list VPS4B, CHMP2A and IST1 as VTA1 binding partners. |
HostingRepository | PRIDE |
AnnounceDate | 2018-02-23 |
AnnouncementXML | Submission_2018-02-23_04:24:28.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Mueller |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-07 08:19:26 | ID requested | |
⏵ 1 | 2018-02-23 04:24:29 | announced | |
2 | 2024-10-22 04:42:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
Staubach S, Wenzel A, Beck BB, Rinschen MM, M, ü, ller S, Hanisch FG, Autosomal Tubulointerstitial Kidney Disease-MUC1 Type: Differential Proteomics Suggests that Mutated MUC1 (insC) Affects Vesicular Transport in Renal Epithelial Cells. Proteomics, 18(7):e1700456(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: ADTKD-MUC1 / MDCK1 / vesicular transport / multivesicular bodies, exosomes |
Contact List
Franz-Georg Hanisch |
contact affiliation | Institute of Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931 Köln, Germany; Center for Molecular Medicine Cologne, University of Cologne, Robert-Koch-Str. 21, 50931 Köln, Germany |
contact email | franz.hanisch@uni-koeln.de |
lab head | |
Stefan Mueller |
contact affiliation | Center for Molecular Medicine Cologne (CMMC) |
contact email | stefan.mueller@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008389
- Label: PRIDE project
- Name: MUC1(insC) affects vesicular transport in renal epithelial cells