PXD008378-1

PXD008378 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of CD34+ CD123+ stem cells from patients with acute myeloid leukemia
Description	The comparative characterization of hematopoietic stem cells from healthy stem cell donors and patients with acute myeloid leukemia on a proteome level has the potential to reveal differentially regulated proteins which might be candidates for specific immunotherapy target molecules. Exemplarily, we analyzed the proteome of the cytosolic and the membrane fraction of CD34 and CD123 co-expressing FACS-sorted leukemic progenitors from five patients with acute myeloid leukemia employing mass spectrometry. As a reference, CD34+CD123+ normal hematopoietic progenitor cells from five healthy stem cell donors were analyzed. In this TMT 10-plex labeling based approach 2068 proteins were identified with 256 proteins differentially regulated in one or both cellular compartments. This study demonstrates the feasibility of a mass spectrometry based proteomic approach to detect differentially expressed proteins in two compartment fractions of leukemic stem cells as compared to their healthy stem cell counterparts.
HostingRepository	PRIDE
AnnounceDate	2018-02-19
AnnouncementXML	Submission_2018-02-19_02:46:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes R. Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-06 05:59:44	ID requested
⏵ 1	2018-02-19 02:46:12	announced

Schmidt JR, R, ü, cker-Braun E, Heidrich K, von Bonin M, St, ö, lzel F, Thiede C, Middeke JM, Ehninger G, Bornh, ä, user M, Schetelig J, Schubert K, von Bergen M, Heidenreich F, Progenitor Cells for the Identification of Potential Targets for Immunotherapy in Acute Myeloid Leukemia. Proteomes, 6(1):(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: acute myeloid leukemia, AML, LC-MS, proteomics

Martin von Bergen
contact affiliation	Department for Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Johannes R. Schmidt
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	johannes.schmidt@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008378

PRIDE project URI

• PRIDE
  1. PXD008378
     1. Label: PRIDE project
     2. Name: Proteomic characterization of CD34+ CD123+ stem cells from patients with acute myeloid leukemia