PXD008372-3

PXD008372 is an original dataset announced via ProteomeXchange.

Title	Processing and Structure of the New Lantibiotic Peptide Nso From the Human GI Tract Bacterium Blautia obeum A2-162 analysed by Mass Spectrometry
Description	A previously reported gene cluster encoding four nisin-like peptides, three with the same sequence (NsoA1-3) and the unique NsoA4, produced antimicrobial activity in the presence of trypsin after heterologous expression in Lactococcus lactis. Protein extracts were prepared by SDS gel electrophoresis or immunoprecipitation using an antibody to the NsoA2 leader. The tryptic peptides observed by LC-MS/MS covered the complete sequence of preNsoA1-3 and part of the leader sequence of preNsoA4 and confirmed the expression and the predicted sequences of the preNsoA peptides. Further, the data revealed that the preNsoA1-3 peptides were partly modified with dehydrations and formation of lanthionine rings. A certain amount of fully modified preNsoA1-3 was observed. Details of modifications of the core peptide and the C-terminal tryptic peptide TATCGCHITGK covering rings D and E indicated that 22% of these preNsoA1-3 peptides were completely modified. A lower amount is estimated for rings A-C. Intact masses of immunoprecipitation derived peptides determined by LC-MS accurately matched the expected preNsoA precursor peptides. The most abundant peptides detected were preNsoA2-3-8H2O followed by preNsoA1-8H2O and other states of dehydration. The results indicate that processing occurs but is incomplete for preNsoA peptides in the heterologous system with the formation of a certain amount of fully modified and active peptides.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:35.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Blautia obeum; NCBI TaxID: 40520;
ModificationList	dehydrated residue; lanthionine; 3-methyllanthionine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-05 08:04:55	ID requested
1	2018-07-04 06:43:16	announced
2	2018-07-12 09:30:38	announced	Updated publication reference for PubMed record(s): 29973605.
⏵ 3	2024-10-22 04:42:36	announced	2024-10-22: Updated project metadata.

10.1038/s41598-018-28248-6;

Gherghisan-Filip C, Saalbach G, Hatziioanou D, Narbad A, Mayer MJ, Processing and Structure of the Lantibiotic Peptide Nso From the Human Gut Bacterium Blautia obeum A2-162 analysed by Mass Spectrometry. Sci Rep, 8(1):10077(2018) [pubmed]

curator keyword: Biological

submitter keyword: lachnospiraceae, dehydration,gut pathogens, antimicrobial peptide, mass spectrometry, nisin, structure, nso, lantibiotic, modification, methyllanthionine ring

Melinda J. Mayer
contact affiliation	Quadram Institute Bioscience, Department of Gut Health and Food Safety, Norwich Research Park, Norwich, NR4 7UA, UK Phone: +44 (0)1603 255284
contact email	melinda.mayer@quadram.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008372

PRIDE project URI

• PRIDE
  1. PXD008372
     1. Label: PRIDE project
     2. Name: Processing and Structure of the New Lantibiotic Peptide Nso From the Human GI Tract Bacterium Blautia obeum A2-162 analysed by Mass Spectrometry