PXD008365-2

PXD008365 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Identify a Novel DNA Repair-Dependent Role for Hepatocyte Nuclear Factor 4α
Description	Hepatocyte nuclear factor 4 (HNF4) is a transcription factor that acts as a master regulator of genes in several endodermal-derived tissues, including the intestine in which it plays a central role during development and tumorigenesis. To better delineate the mechanisms by which HNF4 can interfere during these processes, we combined stable isotope labelling with amino acids in cell culture (SILAC)-based quantitative proteomics with immunoprecipitation of green fluorescent protein (GFP) as well as with proximity-dependent purification by the biotin ligase BirA, both fused to HNF4. Surprisingly, these analyses identified a significant enrichment of proteins falling into the DNA repair gene ontology term, a so far unidentified biological feature of this transcription factor. Several of these proteins including PARP1, RAD50 and DNA-PKcs were confirmed to interact with HNF4in colorectal cancer cell lines. During DNA damage response, HNF4 localized to double strand DNA breaks in these cells. HNF4was able to interfere functionally during non-homologous end-joining (NHEJ). Overall, these observations identify an unsuspected role for this transcription factor during the DNA damage response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:29.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samuel Wilson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-05 02:00:29	ID requested
1	2019-11-12 10:21:37	announced
⏵ 2	2024-10-22 04:42:31	announced	2024-10-22: Updated project metadata.

10.3390/cancers11050626;

Babeu JP, Wilson SD, Lambert É, L, é, vesque D, Boisvert FM, Boudreau F, in Colorectal Cancer Cells. Cancers (Basel), 11(5):(2019) [pubmed]

curator keyword: Biological

submitter keyword: GFP-Trap,colorectal cancer, DNA repair, human, SILAC, HNF4alpha, quantitative proteomics, BioID

François Boudreau
contact affiliation	Department of Anatomy and Cell Biology Faculty of Medicine and Health Science Université de Sherbrooke
contact email	Francois.Boudreau@USherbrooke.ca
lab head
Samuel Wilson
contact affiliation	Université de Sherbrooke
contact email	samuel.wilson@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD008365

PRIDE project URI

• PRIDE
  1. PXD008365
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Identify a Novel DNA Repair-Dependent Role for Hepatocyte Nuclear Factor 4α