PXD008343 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38 |
Description | CRISPR/Cas9-mediated gene-editing allows manipulation of a gene of interest in its own chromosomal context. When applied to the analysis of protein interaction, and in contrast to an exogenous expression of a protein, these can be studied maintaining physiological stochiometries, topology and context. We have used CRISPR/Cas9-mediated recombination to Cluap1/ IFT38, a component of the intraflagellar transport complex B (IFT-B). Cluap1 has been implicated in human development as well as in cancer progression. Cluap1 loss of function results in early developmental defects with neural tube closure, sonic hedgehog signaling and left-right defects. Herein, we generated an endogenously tagged Cluap1 for protein complex analysis which was then correlated to the corresponding interactome determined by ectopic expression. |
HostingRepository | PRIDE |
AnnounceDate | 2018-04-06 |
AnnouncementXML | Submission_2018-04-10_01:11:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tina Beyer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-01 00:29:20 | ID requested | |
1 | 2018-04-04 06:07:58 | announced | |
2 | 2018-04-06 00:38:59 | announced | Updated project metadata. |
⏵ 3 | 2018-04-10 01:11:04 | announced | Updated project metadata. |
Publication List
Beyer T, Bolz S, Junger K, Horn N, Moniruzzaman M, Wissinger Y, Ueffing M, Boldt K, CRISPR/Cas9-mediated Genomic Editing of Cluap1/IFT38 Reveals a New Role in Actin Arrangement. Mol Cell Proteomics, 17(7):1285-1294(2018) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: CRISPR/Cas9, cilia, IFT, proteomics |
Contact List
Marius Ueffing |
contact affiliation | Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Germany |
contact email | Marius.Ueffing@uni-tuebingen.de |
lab head | |
Tina Beyer |
contact affiliation | University Clinics Tuebingen |
contact email | tina.beyer@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008343
- Label: PRIDE project
- Name: CRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38