PXD008313 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen |
Description | The absolute amount of plastocyanin (PC), ferredoxin-NADP+-oxidoreductase (FNR), hydrogenase (HYDA1) and ferredoxin 5 (FDX5) were quantified in aerobic and anaerobic Chlamydomonas reinhardtii whole cells using purified (recombinant) proteins as internal standard in a mass spectrometric approach. Quantified protein amounts were related to the estimated amount of PSI. The ratios of PC to FNR to HYDA1 to FDX5 in aerobic cells were determined to be 2.1 : 1.8 : 0.005 : 0. In anaerobic cells, the ratios changed to 1.6 : 1.8 : 0.026 : 0.04 (PC : FNR : HYDA1 : Fdx5). Employing sodium dithionite and methyl viologen as electron donors the specific activity of hydrogenase in whole cells was calculated to be 382 ±96.5 μmolH2/min/mg. Importantly, these data demonstrate for competition of electrons from FDX1, HYDA1 is about 70-fold less abundant than its competitor FNR. Effective in vivo hydrogen production points to a channeling mechanism for FDX to HYDA1 electron transfer. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-23 |
AnnouncementXML | Submission_2022-02-23_08:42:19.342.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Denitsa Nikolova |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-28 05:58:49 | ID requested | |
⏵ 1 | 2022-02-23 08:42:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: plastocyanin, hydrogenase, ferredoxin-NADP+-oxidoreductase, absolute quantification, ferredoxin 5, Chlamydomonas reinhardtii |
Contact List
Michael Hippler |
contact affiliation | University Münster, Institute for Biology and Biotechnology of Plants |
contact email | mhippler@uni-muenster.de |
lab head | |
Denitsa Nikolova |
contact affiliation | University of Münster |
contact email | dennitsa.nikolova@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008313
- Label: PRIDE project
- Name: Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen