PXD008277-2

PXD008277 is an original dataset announced via ProteomeXchange.

Title	Proteome and phosphoproteome of Wip1-knockout mouse testis
Description	Spermatogenesis plays an important role in the mammalian testis, involving in the complex processes of mitosis, meiosis, and spermiogenesis. Spermatogenesis may also be disrupted in the absence of the immunological and ‘fence’ functions of the BTB, resulting in male subfertility or infertility. Mice lacking wild-type p53-induced phosphatase 1 (Wip1) display male reproductive organ defects, but the molecular mechanisms underlying these abnormalities remain unclear. We explored the function of Wip1 in spermatogenesis and fertility by examining differences in the expressed testis proteome and phosphoproteome between Wip1-deficient and wild-type mice using a proteomics approach. 90 proteins and 178 phosphoproteins were differentially regulated between these two groups of mice. These results suggested that proinflammatory cytokines may impair the blood–testis barrier dynamics by decreasing the expression of junction-associated proteins, which effect could be partially responsible for the subfertility and spermatogenesis defects in Wip1-knockout mice.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:19.582.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yinghui wei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-23 01:18:34	ID requested
1	2022-02-28 19:54:07	announced
⏵ 2	2024-10-22 05:34:20	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Fertility,Proteomic, Phosphoproteomic, Knockout mouse, Wip1, Testis

Yinghui Wei
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	yinghuiwei1019@126.com
lab head
yinghui wei
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	yinghuiwei1019@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008277
     1. Label: PRIDE project
     2. Name: Proteome and phosphoproteome of Wip1-knockout mouse testis