PXD008231-1

PXD008231 is an original dataset announced via ProteomeXchange.

Title	Paraquat mediated oxidative stress in Anopheles gambiae mosquitoes is regulated by an endoplasmic reticulum (ER) stress response
Description	A deeper understanding of malaria parasite development inside the Anopheles mosquito may lead to the identification of processes that can be targeted by transmission-blocking interventions. Paraquat (1,1'-dimethyl-4,4'-bipyridylium dichloride) is a potent superoxide-inducing agent that impacts Plasmodium ookinete development, especially at higher concentrations. Compounds like Paraquat can potentially induce an oxidative imbalance in the mosquito midgut during ookinete maturation, essentially super-stressing the parasite leading to the arrested development of ookinetes, the only stage that can invade through a mosquito midgut cell to establish an oocyst infection in the mosquito. The mosquito midgut has evolved to handle the natural production of reactive oxygen and nitrogen species (ROS and RNS, respectively) as a result of feeding on blood. The addition of Paraquat to a bloodmeal is expected to induce a cognate response in the midgut to handle the excess ROS/RNS, and high concentrations of this compound can potentially overwhelm the midgut response leading to mosquito death. While several studies have explored the effect of Paraquat on malaria parasites, a fundamental understanding of the mosquito response to this compound remains unknown. Here, we quantified the mosquito midgut proteomic response to a Paraquat-laced sugar meal to understand the intrinsic midgut response (in the absence of a bloodmeal). We then carried out transcriptomic analysis of the mosquito midgut for several antioxidants of the Trx and GSH pathways to compare concordance or discordance between protein and its transcripts during different oxidative stress conditions. Finally, we determined whether the same Trx and GSH pathways are upregulated following infection with either P. falciparum or P. berghei at 24 hrs post-blood feeding, coinciding with the time point for maximal ookinete traversal of the midgut. We discuss the potential selective action of Paraquat on the parasite and the intrinsic tolerance of the mosquito midgut to Paraquat-mediated oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:19:07.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chun Hin Law
SpeciesList	scientific name: Anopheles gambiae (African malaria mosquito); NCBI TaxID: 7165;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-17 08:36:41	ID requested
⏵ 1	2024-10-08 11:19:07	announced

10.3390/PROTEOMES6040047;

curator keyword: Biological

submitter keyword: Malaria

Anopheles gambiae

oxidative stress

endoplasmic reticulum stress

Paraquat

transmission-blocking.

Dr. Rhoel Dinglasan
contact affiliation	Emerging Pathogens Institute, University of Florida
contact email	rdinglasan@epi.ufl.edu
lab head
Chun Hin Law
contact affiliation	University of Nebraska Medical Center
contact email	henry.law@unmc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008231
     1. Label: PRIDE project
     2. Name: Paraquat mediated oxidative stress in Anopheles gambiae mosquitoes is regulated by an endoplasmic reticulum (ER) stress response