PXD008223 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CX-MS of TFIIH and Def1 complex |
Description | The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans,Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response tomultiple stimuli, yet transcription activity has not been examined. We have found that Def1 copurifies from yeast whole-cell extract with TFIIH,the largest general transcription factor required for transcription initiation and nucleotide excision repair. The addition of recombinant Def1 and Ela1-Elc1 enhanced transcription initiation in an in vitro reconstituted system including pol II, the general transcription factors, and TFIIS. Def1 also enhanced transcription restart from TFIIS-induced cleavage in a pol II transcribing complex. In the Δdef1 strain, heat shock genes were misregulated, indicating that Def1 is required for induction of some stress-responsive genes in yeast. Taken together, our results extend the understanding of the molecular mechanism of transcription regulation on cellular stress and reveal functional similarities to the mammalian system. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-22 |
AnnouncementXML | Submission_2018-10-22_10:21:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nir Kalisman |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-16 01:20:32 | ID requested | |
⏵ 1 | 2018-10-22 10:21:25 | announced | |
Publication List
Damodaren N, Van Eeuwen T, Zamel J, Lin-Shiao E, Kalisman N, Murakami K, Def1 interacts with TFIIH and modulates RNA polymerase II transcription. Proc Natl Acad Sci U S A, 114(50):13230-13235(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: transcription, RNA polymerase II, TFIIH,stress response, Def1 |
Contact List
Nir Kalisman |
contact affiliation | Dept. of Biological Chemistry, The Hebrew University of Jerusalem |
contact email | nirka@mail.huji.ac.il |
lab head | |
Nir Kalisman |
contact affiliation | Dept. of Biological Chemistry, The Hebrew University of Jerusalem |
contact email | nirka@mail.huji.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008223 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008223
- Label: PRIDE project
- Name: CX-MS of TFIIH and Def1 complex