PXD008214-2

PXD008214 is an original dataset announced via ProteomeXchange.

Title	Host-pathogen dynamics through secretome analysis of stimulated macrophages
Description	The Toll-like receptors (TLRs) recognize different pathogen associated molecular patterns (PAMPs) and promote MyD88 dependent and independent responses. Previously, we have reported the discovery of the temporal changes in signaling cascades of macrophage proteome and secretome post-stimulation with different PAMPs. Here we present strategies to profile the secretome of TLR2-, TLR4, and TLR7- stimulated macrophages using whole pathogens were developed. Stable isotope labeling with amino acids in cell culture of macrophages was integrated with whole pathogen macrophage stimulation and subsequent targeted proteomics to quantify cytokines, chemokines, and transcription factors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:12.824.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-15 01:23:20	ID requested
1	2022-02-28 03:52:38	announced
⏵ 2	2024-10-22 05:34:13	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: TLR4, macrophage, TLR2, TLR7,Secretome, targeted proteomics, LC-MS/MS

Aleksandra Nita-Lazar
contact affiliation	Investigator Chief, Cellular Networks Proteomics Unit Laboratory of Immune System Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg. 4 Rm. 101 4 Memorial Dr Bethesda MD 20892-1892
contact email	nitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD008214

PRIDE project URI

• PRIDE
  1. PXD008214
     1. Label: PRIDE project
     2. Name: Host-pathogen dynamics through secretome analysis of stimulated macrophages