PXD008213-1

PXD008213 is an original dataset announced via ProteomeXchange.

Title	KMT2A-rearranged leukemia proteomics
Description	Activating mutations in kinase/PI3K/RAS signaling pathways are common in acute leukemia with KMT2A rearrangements (KMT2A-R). These mutations are often subclonal and their biological impact remain unclear. Using a retroviral acute myeloid leukemia model, we demonstrate that NRASG12D, FLT3ITD, and FLT3N676K accelerates KMT2A-MLLT3 leukemia onset. Importantly, also the presence of subclonal FLT3N676K in KMT2A-R leukemic cells shorten disease latency, possibly by providing stimulatory factors such as Mif. Acquired de novo mutations in Braf, Cbl, Kras, and Ptpn11 were identified in KMT2A-MLLT3 driven leukemia and favored clonal expansion. KMT2A-MLLT3 leukemia with an activating mutation enforce Myc- and Myb transcriptional modules, whereas KMT2A-MLLT3 leukemias lacking activating mutations displayed upregulation of signal transduction pathways. Our results provide new insight into the biology of KMT2A-R leukemia and highlights the importance of activated signaling as a contributing driver in this disease.
HostingRepository	PRIDE
AnnounceDate	2018-05-02
AnnouncementXML	Submission_2018-05-01_23:46:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Hansson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-15 01:21:20	ID requested
⏵ 1	2018-05-01 23:46:20	announced
2	2018-05-07 00:04:22	announced	Updated publication reference for PubMed record(s): 29720585.
3	2018-05-07 00:59:19	announced	Updated project metadata.
4	2019-03-06 02:11:22	announced	Updated project metadata.
5	2024-10-22 04:41:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Mouse, leukemia, TMT

Jenny Hansson
contact affiliation	Division of Molecular Hematology, Department of Laboratory Medicine, Lund University
contact email	jenny.hansson@med.lu.se
lab head
Jenny Hansson
contact affiliation	Lund University
contact email	jehansson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008213

PRIDE project URI

• PRIDE
  1. PXD008213
     1. Label: PRIDE project
     2. Name: KMT2A-rearranged leukemia proteomics