PXD008205-1

PXD008205 is an original dataset announced via ProteomeXchange.

Title	Contractile work contributes to maturation of energy metabolism in hiPSC-derived cardiomyocytes
Description	Energy metabolism is a key aspect of cardiomyocyte biology. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are a promising tool for biomedical application, but they are immature and have not undergone metabolic shift related to early postnatal development. Cultivation of hiPSC-CM in 3D engineered heart tissue (EHT) format leads to morphological maturation. This study compared the mitochondrial and metabolic state of hiPSC-CM in standard 2D culture and the EHT format and determined the influence of contractile activity. HiPSC-CM in EHTs showed ~2-fold higher number of mitochondria (electron microscopy), mitochondrial mass (mitotracker), DNA (Mt-ND1, Mt-ND2), and protein abundance (proteome) than in 2D culture. While hiPSC-CM exhibited the principal ability to use glucose, lactate and fatty acids as energy substrates irrespective of culture format, hiPSC-CM in 3D performed more oxidation of glucose, lactate and fatty acid, and less anaerobic glycolysis. The increase in mitochondrial mass and DNA in 3D was diminished by pharmacological inhibition of contractile force, suggesting that contractile work participates in mitochondrial development hiPSC-CM. In conclusion, contractile work in the EHT format contributes to metabolic maturation of hiPSC-CM.
HostingRepository	PRIDE
AnnounceDate	2018-03-06
AnnouncementXML	Submission_2018-03-06_04:46:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008205
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Stoehr
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-14 01:21:59	ID requested
⏵ 1	2018-03-06 04:46:29	announced
2	2024-10-22 04:42:20	announced	2024-10-22: Updated project metadata.

Ulmer BM, Stoehr A, Schulze ML, Patel S, Gucek M, Mannhardt I, Funcke S, Murphy E, Eschenhagen T, Hansen A, Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes. Stem Cell Reports, 10(3):834-847(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, iPSC, cardiomyocyte, engineered heart tissue

Arne Hansen
contact affiliation	Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	ar.hansen@uke.de
lab head
Andrea Stoehr
contact affiliation	Karolinska Institutet
contact email	andrea.stoehr@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008205
     1. Label: PRIDE project
     2. Name: Contractile work contributes to maturation of energy metabolism in hiPSC-derived cardiomyocytes