PXD008193-3

PXD008193 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A method for producing heat-stable and pure trypsin
Description	In this study we evaluated several different strategies for reductive methylation and purification of porcine trypsin in order to write a protocol that can be followed by proteomics laboratories.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:06.152.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Søren Heissel
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-13 01:51:14	ID requested
1	2019-06-06 05:41:54	announced
2	2019-07-09 04:49:28	announced	Updated publication reference for PubMed record(s): 31246970.
⏵ 3	2024-10-22 04:42:10	announced	2024-10-22: Updated project metadata.

Publication List

10.1371/journal.pone.0218374;
Heissel S, Frederiksen SJ, Bunkenborg J, H, ø, jrup P, Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications. PLoS One, 14(6):e0218374(2019) [pubmed]

10.1371/journal.pone.0218374;

Heissel S, Frederiksen SJ, Bunkenborg J, H, ø, jrup P, Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications. PLoS One, 14(6):e0218374(2019) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: Trypsin, Dimethylation, LC-MS

curator keyword: Technical

submitter keyword: Trypsin, Dimethylation, LC-MS

Contact List

Peter Højrup
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, DK
contact email	php@bmb.sdu.dk
lab head
Søren Heissel
contact affiliation	University of Southern Denmark
contact email	sheissel@bmb.sdu.dk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD008193
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD008193

PRIDE project URI

Repository Record List

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