PXD008183-1

PXD008183 is an original dataset announced via ProteomeXchange.

Title	Human Ovarian Proteome
Description	Fertility preservation research in women today is increasingly taking advantage of bioengineering techniques to develop new biomimetic materials and solutions to safeguard ovarian cell function and microenvironment in vitro and in vivo. However, available data on the human ovary are limited and fundamental differences between animal models and humans are hampering researchers in their quest for more extensive knowledge of human ovarian physiology and key reproductive proteins that need to be preserved. We therefore turned to multi-dimensional label-free mass spectrometry to analyze human ovarian cortex, as it is a high-throughput and conclusive technique providing information on the proteomic composition of complex tissues like the ovary. In-depth proteomic profiling through two-dimensional liquid chromatography-mass spectrometry, western blot, histological and immunohistochemical analyses, and data mining helped us to confidently identify 1,508 proteins. Moreover, our method allowed us to chart the most complete representation so far of the ovarian matrisome, defined as the ensemble of extracellular matrix proteins and associated factors, including more than 80 proteins. In conclusion, this study will provide a better understanding of ovarian proteomics, with a detailed characterization of the ovarian follicle microenvironment, in order to enable bioengineers to create biomimetic scaffolds for transplantation and three-dimensional in vitro culture. By publishing our proteomic data, we also hope to contribute to accelerating biomedical research into ovarian health and disease in general
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_10:25:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-10 05:36:30	ID requested
⏵ 1	2018-02-27 10:25:46	announced

Ouni E, Vertommen D, Chiti MC, Dolmans MM, Amorim CA, A Draft Map of the Human Ovarian Proteome for Tissue Engineering and Clinical Applications. Mol Cell Proteomics, 18(Suppl 1):S159-S173(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Ovarian, Matrisome, proteome

Christiani Andrade Amorim
contact affiliation	Université catholique de Louvain Institut de recherche expérimentale et clinique (IREC) GYNE Avenue Mounier 52/B1.52.02 1200 Woluwe-Saint-Lambert Belgium
contact email	christiani.amorim@uclouvain.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008183

PRIDE project URI

• PRIDE
  1. PXD008183
     1. Label: PRIDE project
     2. Name: Human Ovarian Proteome