PXD008182-1

PXD008182 is an original dataset announced via ProteomeXchange.

Title	Adaptation of CysPAT technique to characterize endogenous S-nitrosylation in RAW264.7 cells
Description	The aim of the study was to adapt the CysPAT technique to efficiently identify and characterize endogenous S-nitrosylation in cells under physiological conditions of an experiment from a small amount of sample. Using a macrophage cell line RAW 264.7 we compared the efficacy of the method in the identification of total endogenous S-nitrosylation and the corresponding modification of the samples treated with exogenous SNO donor (GSNO) or with DTT. We identified 999 unique formerly S-nitrosylated peptides (SIA peptides) and 965 unique SNO sites from untreated RAW cells which belong to 569 endogenous nitrosylated proteins. We discovered 579 novel S-nitrosylation sites and identified 232 proteins as new S-nitrosylation targets. A total of 1450 S-nitrosylated peptides belonging to 795 proteins were identified in samples treated with GSNO (n = 3). Interestingly, we found a large overlap (>53%) of S-nitrosylated peptides in both subsets of samples, demonstrating a high sensitivity of the method to analyze endogenous S-nitrosylation. The large number of identified endogenous S-nitrosylated peptides allowed the identification of two nitrosylation consensus sites, and to highlight protein translation and redox processes as key S-nitrosylation targets in macrophages.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:46:47.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Ibañez-Vea
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-ethylmaleimide derivatized cysteine; phosphorylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-10 05:36:02	ID requested
⏵ 1	2022-02-22 03:46:48	announced

Ib, á, ñ, ez-Vea M, Huang H, Mart, í, nez de Morentin X, P, é, rez E, Gato M, Zuazo M, Arasanz H, Fern, á, ndez-Irigoyen J, Santamar, í, a E, Fernandez-Hinojal G, Larsen MR, Escors D, Kochan G, Chromatography. J Proteome Res, 17(3):1172-1182(2018) [pubmed]

curator keyword: Biological

submitter keyword: Proteomics, post-translational modifications (PTMs), S-nitrosylation, macrophages, immune system

David Escors
contact affiliation	Immunomodulation Group, Navarrabiomed Biomedical Research Center, Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Spain
contact email	david.escors.murugarren@navarra.es
lab head
Maria Ibañez-Vea
contact affiliation	Navarrabiomed Biomedical Research Center
contact email	maria.ibanez.vea@navarra.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008182
     1. Label: PRIDE project
     2. Name: Adaptation of CysPAT technique to characterize endogenous S-nitrosylation in RAW264.7 cells