PXD008112-2

PXD008112 is an original dataset announced via ProteomeXchange.

Title	Interleukin-2 shapes the cytotoxic T cell proteome and immune-environment sensing
Description	Interleukin-2 (IL-2) and Janus kinases (JAKs) regulate transcriptional programs and protein synthesis to control the differentiation of effector CD8+ cytotoxic T cells (CTL). Using high-resolution mass spectrometry, we have generated an in-depth characterisation of how IL-2 and JAKs configure the CTL proteome to control CTL function. We found that IL-2-JAK1/3 signaling selectively regulated the abundance of a key subset of proteins influencing the accumulation of critical cytokines and effector molecules in T cells. Moreover, IL-2 controlled the concentration of proteins that support core metabolic processes essential for cellular fitness. One fundamental insight was the dominant role for IL-2 in controlling how effector T cells respond to their microenvironment. IL-2-JAK1/3 signaling pathways thus controlled the abundance of nutrient transporters, nutrient sensors and critical oxygen sensing molecules. The data provide key insights of how IL-2 controls T cell function and highlight signaling mechanisms and transcription factors that link oxygen sensing to transcriptional control of CD8+ T cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2018-04-17
AnnouncementXML	Submission_2018-04-25_06:26:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Ross
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-acetylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-02 02:43:02	ID requested
1	2018-04-17 01:49:29	announced
⏵ 2	2018-04-25 06:26:47	announced	Updated publication reference for PubMed record(s): 29666307.
3	2024-10-22 04:41:37	announced	2024-10-22: Updated project metadata.

Rollings CM, Sinclair LV, Brady HJM, Cantrell DA, Ross SH, Interleukin-2 shapes the cytotoxic T cell proteome and immune environment-sensing programs. Sci Signal, 11(526):(2018) [pubmed]

curator keyword: Biological

submitter keyword: IL-2, Interleukin-2, JAK1, JAK3, proteomics, lymphocytes

Doreen Ann Cantrell
contact affiliation	Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK.
contact email	d.a.cantrell@dundee.ac.uk
lab head
Sarah Ross
contact affiliation	University of Dundee
contact email	s.t.ross@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008112

PRIDE project URI

• PRIDE
  1. PXD008112
     1. Label: PRIDE project
     2. Name: Interleukin-2 shapes the cytotoxic T cell proteome and immune-environment sensing