PXD008082-1

PXD008082 is an original dataset announced via ProteomeXchange.

Title	Optimized NHS-amine labeling for multiplexed phosphoproteome study.
Description	In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate that vacuum-drying in presence of hydroxylamine promotes β-elimination of phosphate groups from phosphoserine and phosphothreonine.
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_08:51:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yumi Kwon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-31 02:29:17	ID requested
⏵ 1	2018-02-27 08:52:00	announced
2	2024-10-22 04:41:43	announced	2024-10-22: Updated project metadata.

Kwon Y, Ju S, Kaushal P, Lee JW, Lee C, Neutralizing the Detrimental Effect of an N-Hydroxysuccinimide Quenching Reagent on Phosphopeptide in Quantitative Proteomics. Anal Chem, 90(5):3019-3023(2018) [pubmed]

curator keyword: Technical

submitter keyword: hydroxylamine, phosphoproteome, phosphorylation, β-elimination, N-hydroxysuccinamide-esters, tandem mass tag, isobaric tag

Cheolju Lee
contact affiliation	Center for Theragnosis, Korea Institute of Science and Technology, Seoul 02792, Korea
contact email	clee270@kist.re.kr
lab head
Yumi Kwon
contact affiliation	Korea Institute of Science and Technology
contact email	ymkwon@kist.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008082

PRIDE project URI

• PRIDE
  1. PXD008082
     1. Label: PRIDE project
     2. Name: Optimized NHS-amine labeling for multiplexed phosphoproteome study.