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PXD008075-2

PXD008075 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell wall enrichment unveils proteomic changes in the cell wall during treatment of Mycobacterium smegmatis with sub-lethal concentrations of Rifampicin
DescriptionIn this study, we successfully developed two gel-free approaches to specifically look at cell wall proteins in Mycobacterium smegmatis. The cell wall was subjected to differential centrifugation, differential detergent solubilisation and phase separation to yield the genuine cell wall proteome. The protein extracts were digested by filter-assisted sample preparation for LC-MS/MS analysis on a Q-Exactive mass spectrometer and identified proteins subjected to a strict bioinformatics pipeline. This resulted in the unprecedented coverage of 96 lipoproteins, 475 membrane proteins containing at least one transmembrane helix and 73 secreted proteins. We used this gel-free approach to study the changes in the cell wall proteome during exposure of M. smegmatis to sub-lethal concentration of Rifampicin in a quantitative manner. This enabled us to characterise in detail the dysregulation of transporters such as TatB and ABC transporters, virulence factors such as MCE proteins and PknG, as well as proteins involved in cell wall and lipid synthesis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:41:17.005.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexander Giddey
SpeciesList scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-30 04:17:30ID requested
12019-04-09 08:39:41announced
22024-10-22 04:41:25announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.jprot.2018.02.019;
Hermann C, Giddey AD, Nel AJM, Soares NC, Blackburn JM, Cell wall enrichment unveils proteomic changes in the cell wall during treatment of Mycobacterium smegmatis with sub-lethal concentrations of rifampicin. J Proteomics, 191():166-179(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Cell wall proteomics, Mycobacteria, Mass spectrometry, Rifampicin, TB drugs, TB, sub lethal
Contact List
Jonathan M Blackburn
contact affiliationDivision of Chemical and Systems Biology, Department of Integrative Biomedical Science, Institute for Infectious Disease and Molecular Medicine, Faculty of Health Science, University of Cape Town, South Africa.
contact emailjonathan.blackburn@uct.ac.za
lab head
Alexander Giddey
contact affiliationUniversity of Cape Town
contact emailgddale001@myuct.ac.za
dataset submitter
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