PXD008033 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chromatin protein profiling during the mammalian cell cycle reveals the extent of mitotic preservation of the regulatory landscape |
Description | While regulation of transcription, replication and cell division relies on dynamic protein binding to DNA and chromatin, it is unclear which regulatory components remain bound to compacted mitotic chromosomes. To comprehensively quantify the chromatin-associated proteome in different phases of the cell cycle, we utilized the buoyant density of DNA-protein complexes after crosslinking. This revealed variable associations for thousands of proteins to DNA including their frequent and variable phosphorylation. While epigenetic modifiers that promote transcription are lost from mitotic chromatin, repressive modifiers generally remain associated. Interestingly, while proteins involved in transcriptional elongation are evicted, most identified transcription factors are retained on mitotic chromatin to varying degrees, including core promoter binding proteins. This predicts conservation of the regulatory landscape on mitotic chromosomes, which genome-wide measurements of chromatin accessibility confirm. This work establishes a novel approach to study chromatin, provides a comprehensive catalogue of chromatin changes during the cell cycle, and reveals the degree with which the genomic regulatory landscape is maintained through mitosis. |
HostingRepository | PRIDE |
AnnounceDate | 2018-09-06 |
AnnouncementXML | Submission_2019-04-03_04:31:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Ginno |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; N6: N6; acetylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-24 07:16:35 | ID requested | |
1 | 2018-09-05 23:50:01 | announced | |
⏵ 2 | 2019-04-03 04:31:11 | announced | Updated publication reference for PubMed record(s): 30279501. |
Publication List
Ginno PA, Burger L, Seebacher J, Iesmantavicius V, Sch, ü, beler D, Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape. Nat Commun, 9(1):4048(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chromatin, Cell Cycle, Mitotic Bookmarking |
Contact List
Dirk Schübeler |
contact affiliation | Friedrich Miescher Institute for Biomedical Research Epigenetics Department Lab 1.22 |
contact email | dirk.schubeler@fmi.ch |
lab head | |
Paul Ginno |
contact affiliation | FMI |
contact email | paul.ginno@fmi.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008033
- Label: PRIDE project
- Name: Chromatin protein profiling during the mammalian cell cycle reveals the extent of mitotic preservation of the regulatory landscape