PXD008020-1

PXD008020 is an original dataset announced via ProteomeXchange.

Title	Cadmium specific proteomic responses of Pseudomonas aeruginosa san ai
Description	Pseudomonas aeruginosa san ai resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to sub-lethal concentration of cadmium (0.9 mM) adsorbed 75% of added cadmium after 48 hours, while remaining 25% in culture supernatant was adsorbed by exopollysaccaryde, implying a large biosorption potential of leaving biomass and its exopollysaccaryde. Inner cell response on the protein level was investigated by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins in complex protein fractions obtained by size exclusion chromatography used to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 59 proteins were observed as up-regulated in cadmium- amended culture. Almost a third of the total number of up-regulated were metalloproteins. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in cell energy metabolism, amino acid metabolism, cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium- amended culture versus control was found. Our results signify that P. aeruginosa san ai has a great potential for application in bioremediation of cadmium polluted sites.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_01:22:36.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivanka Karadzic
SpeciesList	scientific name: Pseudomonas aeruginosa ATCC 15442; NCBI TaxID: 1424337;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	amaZon Speed

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-23 02:37:50	ID requested
⏵ 1	2022-05-19 01:22:36	announced
2	2024-10-22 05:36:17	announced	2024-10-22: Updated project metadata.

Izrael-, Ž, ivkovi, ć L, Rikalovi, ć M, Gojgi, ć, -Cvijovi, ć G, Kazazi, ć S, Vrvi, ć M, Br, č, eski I, Be, š, koski V, Lon, č, arevi, ć B, Gop, č, evi, ć K, Karad, ž, i, ć I, san ai. RSC Adv, 8(19):10549-10560(2018) [pubmed]

curator keyword: Biological

submitter keyword: Pseudomonas aeruginosa san ai

Ivanka Karadzic
contact affiliation	Faculty of Medicine Department of Chemistry Belgrade Serbia
contact email	ivanka.karadzic@med.bg.ac.rs
lab head
Ivanka Karadzic
contact affiliation	School of Medicine, Department of Chemistry
contact email	ivanka.karadzic@med.bg.ac.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008020
     1. Label: PRIDE project
     2. Name: Cadmium specific proteomic responses of Pseudomonas aeruginosa san ai