PXD008017 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | More is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics |
Description | Metaproteomics is a powerful tool to characterize the structure of microbial communities and the physiology, metabolism and interactions of the species in these communities. Metaproteomics seeks to identify and quantify proteins from microbial communities on a large scale using gel electrophoresis or advanced liquid chromatography (LC) combined with high-resolution, accurate-mass mass spectrometry. To achieve extensive coverage of a metaproteome using shotgun proteomics, the sample complexity has to be decreased, for which a main approach is the on-line separation of peptides using one or more LC dimensions. The aim of this study is to test different 1D- and 2D-LC methods, also in comparison with the standard GeLC (pre-separation of proteins via gel electrophoresis) to find the best approach for analyzing metaproteome samples, using a mock community with 32 species in different abundances. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-13 |
AnnouncementXML | Submission_2019-02-13_06:07:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tjorven Hinzke |
SpeciesList | scientific name: Archaea; NCBI TaxID: 2157; scientific name: Eukaryota (eucaryotes); NCBI TaxID: 2759; scientific name: Viruses; NCBI TaxID: 10239; scientific name: Bacteria; NCBI TaxID: 2; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-23 02:18:43 | ID requested | |
⏵ 1 | 2019-02-13 06:07:45 | announced | |
2 | 2019-03-11 14:37:13 | announced | Updated publication reference for PubMed record(s): 30837968. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Metaproteomics, Technical |
submitter keyword: microbial community |
microbiota |
microbiome |
mock community |
method evaluation |
microbial ecology |
Q Exactive |
liquid chromatography |
GeLC |
Contact List
Manuel Kleiner |
contact affiliation | Department of Geoscience, University of Calgary, Calgary, AB, Canada; Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA |
contact email | manuel_kleiner@ncsu.edu |
lab head | |
Tjorven Hinzke |
contact affiliation | University of Greifswald, Greifswald Intitute of Marine Biotechnology |
contact email | tjorven.hinzke@stud.uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008017
- Label: PRIDE project
- Name: More is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics