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PXD007978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsolation of PfPuf3 protein complex and mass spectrometry (MS) analysis
DescriptionRecent studies of the Puf family of RNA-binding proteins revealed that besides their traditional roles in translational regulation of mRNAs, some Puf proteins are also involved in ribosome biogenesis by binding rRNA. In this study, we report the role of a Puf-like protein (Puf3) in ribosome biogenesis in Plasmodium falciparum and Plasmodium yoelii. Secondary structure prediction suggested that the RNA-binding domain of Puf3 consists of 11 pumilio repeats, similar to human Puf-A/yeast Puf6, which is involved in ribosome biogenesis. Neither pfpuf3 nor pypuf3 could be genetically disrupted, suggesting they may be essential for the intraerythrocytic developmental cycle (IDC). A time-course study of PfPuf3 protein indicated that PfPuf3 was expressed during the entire IDC, with peak expression in early trophozoites. Cellular fractionation of PfPuf3 revealed that it is preferentially partitioned to the nuclear rather than the cytoplasmic fractions, which is consistent with a nuclear localization of PfPuf3::GFP and PyPuf3::GFP as seen by immunofluorescence. Further, we found PfPuf3 co-localized with a well-known nucleolus maker, PfNop1, demonstrating that PfPuf3 is a nucleolar protein. This localization is in contrast to the cytoplasmic localization of PfPuf1 and PfPuf2, but matches the localization of human Puf-A and yeast Puf6. Affinity purification of a C-terminal PTP-tagged variant of PfPuf3 revealed an association with 32 proteins associated with the 60S ribosome, and an enrichment of 28S rRNA and internal transcribed spacer 2. Taken together, these results demonstrate a nucleolar localization of PfPuf3 and suggest an essential function of PfPuf3 in ribosomal biogenesis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaoying Liang
SpeciesList scientific name: Plasmodium falciparum UGK 730.2; NCBI TaxID: 1050258;
ModificationListprotein modification categorized by amino acid modified
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-17 02:32:05ID requested
12018-03-12 02:24:11announced
Publication List
Liang X, Hart KJ, Dong G, Siddiqui FA, Sebastian A, Li X, Albert I, Miao J, Lindner SE, Cui L, Puf3 participates in ribosomal biogenesis in malaria parasites. J Cell Sci, 131(6):(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Plasmodium falciparum
Contact List
Liwang Cui
contact affiliationEntomology Department, Penn State Univeristy
contact emailluc2@psu.edu
lab head
Xiaoying Liang
contact affiliationPSU
contact emailkeaiyingying568@gmail.com
dataset submitter
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Dataset FTP location
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