PXD007956 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Linking microbial community structure and function during the anaerobic digestion of grass |
Description | In this study, microbial communities from triplicate leach-bed anaerobic bioreactors digesting grass were analysed. Each reactor comprised two microbial fractions, one immobilized on grass (biofilm) and the other in a planktonic state present in the leachate. Microbial communities from the two fractions were systematically investigated for community composition and function. This was carried out using DNA, RNA and protein co-extraction. The microbial structure of each fraction was examined using 16S rRNA deep sequencing, while the active members of the consortia were identified using the same approach on cDNA generated from co-extracted RNA samples. Microbial function was investigated using a metaproteomic workflow combining SDS-PAGE and LC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2017-12-07 |
AnnouncementXML | Submission_2017-12-07_05:50:15.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007956 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Aoife Joyce |
SpeciesList | scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-12 03:52:42 | ID requested | |
⏵ 1 | 2017-12-07 05:50:15 | announced | |
2 | 2019-03-07 05:42:11 | announced | Updated publication reference for PubMed record(s): 29619022. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Metaproteomics |
submitter keyword: Anaerobic digestion, Metaproteomics, Microbiome, 16S Illumina |
Contact List
Florence Abram |
contact affiliation | Functional Environmental Microbiology, School of Natural S iences, National University of Ireland, Galway |
contact email | florence.abram@nuigalway.ie |
lab head | |
Aoife Joyce |
contact affiliation | NUI Maynooth |
contact email | feefeeseoighe@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007956
- Label: PRIDE project
- Name: Linking microbial community structure and function during the anaerobic digestion of grass