PXD007955 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of post-translational modifications on Odorant-Binding Protein isoforms from pig olfactory secretome by high-resolution mass spectrometry: O-beta-N-acetylglucosaminylation and phosphorylation |
Description | Odorant-Binding Proteins (OBP) are players of perireceptor events in olfaction. A molecular mechanism explaining their specificity for odors and pheromones has still to be proposed. A new track comes from the analysis of pig olfactory secretome, which is mainly composed of OBP isoforms, generated from 3 gene products by PTM, phosphorylation and O‐β‐N‐acetylglucosaminylation (O-GlcNAcylation), which are unusual for secreted proteins. These diverse isoforms could display different binding properties towards ligands. Before testing such a function, both post-translational modifications, although assessed by specific antibodies, have to be identified by mass spectrometry. We report here for the first time, the identification of phosphorylation and O-GlcNAcylation on peptides coming from trypsin digestion of OBP by ESI-HCD-MS/MS. PEAKS software analysis of raw MS data allowed selecting spectra that were analyzed manually to identify PTM. Four peptides corresponding to two different portions of OBP sequence were modified either by a phosphate group or by a hexNAc moiety. Due to the high energy used in HCD, the data did not allow precise localization of the modified sites. We suggest that these two PTM, by generating multiple isoforms, should extend the binding repertoire of secreted OBPs |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:51:25.173.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fabrice Bray |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | phosphorylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-12 03:27:45 | ID requested | |
1 | 2018-12-20 00:41:04 | announced | |
⏵ 2 | 2023-11-14 06:51:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: 2-Dimensional Gel Electrophoresis,Odorant-binding protein, O-GlcNAc, ESI-HCD-MS/MS, Phosphorylation |
Contact List
Patricia NAGNAN-LE MEILLOUR |
contact affiliation | Glycobiology of olfaction, UMR8576 Unité de glycobiologie structurale et fonctionnelle, France. |
contact email | patricia.le-meillour@univ-lille1.fr |
lab head | |
Fabrice Bray |
contact affiliation | MSAP3290 |
contact email | fabrice.bray@univ-lille1.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007955
- Label: PRIDE project
- Name: Identification of post-translational modifications on Odorant-Binding Protein isoforms from pig olfactory secretome by high-resolution mass spectrometry: O-beta-N-acetylglucosaminylation and phosphorylation