PXD007942 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of H3 Lysine 4 Monomethylation Associated Proteins at Mammalian Enhancers |
Description | Enhancers act to regulate cell type specific gene expression by facilitating the transcription of target genes. In mammalian cells active or primed enhancers are commonly marked by monomethylation of Histone H3 at lysine 4 (H3K4me1) in a cell-type specific manner. Whether and how this histone modification regulates enhancer-dependent transcription programs in mammals has been unclear. In the present study, we conducted SILAC Mass-spec experiments with mono-nucleosomes and identified multiple H3K4me1 associated proteins, including proteins involved in chromatin remodeling. We demonstrate that H3K4me1 augments the association of the chromatin remodeling complex BAF to enhancers in vivo. Furthermore we show that in vitro, H3K4me1 nucleosomes are more efficiently remodeled by the BAF complex. Crystal structures of a BAF component BAF45c further reveal that monomethylation, but not trimethylation, is accommodated in this protein’s H3K4 binding site. Our results suggest that H3K4me1 plays an active role at enhancers by facilitating the binding of the BAF complex and possibly other chromatin regulators. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-23 |
AnnouncementXML | Submission_2018-10-23_14:36:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claudio Ponte de Albuquerque |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; 6x(13)C labeled residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-11 04:02:22 | ID requested | |
⏵ 1 | 2018-10-23 14:36:19 | announced | |
Publication List
Local A, Huang H, Albuquerque CP, Singh N, Lee AY, Wang W, Wang C, Hsia JE, Shiau AK, Ge K, Corbett KD, Wang D, Zhou H, Ren B, Identification of H3K4me1-associated proteins at mammalian enhancers. Nat Genet, 50(1):73-82(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: BAF, monomethylation of Histone H3 at lysine 4 (H3K4me1), and Enhancers |
Contact List
Bing Ren |
contact affiliation | Ludwig Institute for Cancer Research |
contact email | biren@ucsd.edu |
lab head | |
Claudio Ponte de Albuquerque |
contact affiliation | UCSD |
contact email | cpontede@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007942 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007942
- Label: PRIDE project
- Name: Identification of H3 Lysine 4 Monomethylation Associated Proteins at Mammalian Enhancers