PXD007899-1

PXD007899 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Integrated in vivo quantitative proteomics and nutrient tracing reveals age-related metabolic rewiring of pancreatic B-cell function
Description	Aging is associated with fundamental changes in pancreatic B-cell physiology; yet, the mechanisms that drive these age-related changes are poorly understood. We performed comprehensive proteomic profiling of pancreatic islets from adolescent and old mice. The analysis revealed striking differences in abundance of enzymes controlling glucose metabolism not reflected at the transcript level. We show that these changes in protein abundance are associated with increased activity of the amplifying pathway of insulin secretion. The amplifying pathway stimulates insulin secretion through coupling factors produced during glucose metabolism. Nutrient tracing and targeted metabolomics demonstrate accelerated accumulation of glucose-derived metabolites and coupling factors in aged islets, indicating that age-related changes in glucose metabolism contribute to the improved response of B-cells to glucose with age. Together, our study provides the first in-depth characterization of changes in the islet proteome during aging and establishes metabolic rewiring as an important mechanism for age-associated changes in B-cell function.
HostingRepository	MassIVE
AnnounceDate	2018-12-03
AnnouncementXML	Submission_2018-12-03_08:58:43.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvador Martínez de Bartolomé
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	unknown modification: PRIDE:0000398
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-03 11:18:20	ID requested
⏵ 1	2018-12-03 08:58:44	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: aging, SILAM Mud-PIT mass spectrometry, quantitative proteomics, insulin secretion, B-cell, B-cell maturation, B-cell function

submitter keyword: aging, SILAM Mud-PIT mass spectrometry, quantitative proteomics, insulin secretion, B-cell, B-cell maturation, B-cell function

Contact List

Maike Sander
contact affiliation	University California San Diego (UCSD)
contact email	masander@ucsd.edu
lab head
Salvador Martínez de Bartolomé
contact affiliation	The Scripps Research Institute
contact email	salvador@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081577