PXD007862-1

PXD007862 is an original dataset announced via ProteomeXchange.

Title	BIOSITe: A novel method for direct detection of site specific biotinylation
Description	Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family proteins for the capture of biotinylated proteins. While the extremely high affinity of the avidin-biotin interaction nearly guarantees the capture of biotinylated proteins from a sample, it also results in an inability to recover the key species of interest: the biotin-modified peptide. To overcome this limitation, we have developed a novel strategy that instead relies on an immunoaffinity-based capture of biotinylated peptides for downstream LC-MS/MS analysis. We refer to this approach as Biotinylation Site Identification Technology (BioSITe), since it enables the direct detection of site-specific biotinylation, which substantially increases the sensitivity and specificity of data offered by proximity-dependent biotinylation methods. Using isotopically labeled biotin, we also demonstrate that this method can be used for differential analysis. Overall, we anticipate this method to replace the current methods used for detection of biotinylation sites and other applications including those employing any molecule probe for enrichment of specific classes of proteins or post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2017-12-19
AnnouncementXML	Submission_2017-12-19_02:18:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-28 01:21:37	ID requested
⏵ 1	2017-12-19 02:18:19	announced
2	2024-10-22 04:40:59	announced	2024-10-22: Updated project metadata.

Kim DI, Cutler JA, Na CH, Reckel S, Renuse S, Madugundu AK, Tahir R, Goldschmidt HL, Reddy KL, Huganir RL, Wu X, Zachara NE, Hantschel O, Pandey A, BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation. J Proteome Res, 17(2):759-769(2018) [pubmed]

curator keyword: Technical

submitter keyword: BioID, Biotinylation, IP, BCR ABL, peptide, mass spectrometry, proximity dependent biotinylation, BioSITe,

Akhilesh Pandey
contact affiliation	McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
contact email	pandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007862
     1. Label: PRIDE project
     2. Name: BIOSITe: A novel method for direct detection of site specific biotinylation