PXD007852-2

PXD007852 is an original dataset announced via ProteomeXchange.

Title	Analysis of the Salivary Gland Transcriptome of Unfed and Partially Fed Amblyomma sculptum Ticks and Descriptive Proteome of the Saliva
Description	Ticks are obligate blood feeding ectoparasites that transmit a wide variety of pathogenic microorganisms to their vertebrate hosts. Amblyomma sculptum is vector of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, the most lethal rickettsiosis that affects humans. It is known that the transmission of pathogens by ticks is mainly associated with the physiology of the feeding process. Pathogens that are acquired with the blood meal must first colonize the tick gut and later the salivary glands in order to be transmitted during a subsequent blood feeding via saliva. Tick saliva contains a complex mixture of bioactive molecules with anticlotting, antiplatelet aggregation, vasodilatory, anti-inflammatory, and immunomodulatory properties to counteract both the hemostasis and defense mechanisms of the host. Besides facilitating tick feeding, the properties of saliva may also benefits survival and establishment of pathogens in the host. In the current study, we compared the sialotranscriptome of unfed A. sculptum ticks and those fed for 72 h on rabbits using next generation RNA sequencing (RNA-seq). The total of reads obtained were assembled in 9,560 coding sequences (CDSs) distributed in different functional classes. CDSs encoding secreted proteins, including lipocalins, mucins, protease inhibitors, glycine-rich proteins, metalloproteases, 8.9 kDa superfamily members, and immunity-related proteins were mostly upregulated by blood feeding. Selected CDSs were analyzed by real-time quantitative polymerase chain reaction preceded by reverse transcription (RT-qPCR), corroborating the transcriptional profile obtained by RNA-seq. Finally, high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis revealed 124 proteins in saliva of ticks fed for 96-120 h. The corresponding CDSs of 59 of these proteins were upregulated in salivary glands of fed ticks. To the best of our knowledge, this is the first report on the proteome of A. sculptum saliva. The functional characterization of the identified proteins might reveal potential targets to develop vaccines for tick control and/or blocking of R. rickettsii transmission as well as pharmacological bioproducts with antihemostatic, anti-inflammatory and antibacterial activities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:52.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebeca Kawahara
SpeciesList	scientific name: Amblyomma sculptum; NCBI TaxID: 1581419;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-27 02:44:19	ID requested
1	2018-10-23 14:38:01	announced
⏵ 2	2024-10-22 04:39:53	announced	2024-10-22: Updated project metadata.

Esteves E, Maruyama SR, Kawahara R, Fujita A, Martins LA, Righi AA, Costa FB, Palmisano G, Labruna MB, S, á, -Nunes A, Ribeiro JMC, Foga, ç, a AC, Ticks and Descriptive Proteome of the Saliva. Front Cell Infect Microbiol, 7():476(2017) [pubmed]

10.3389/fcimb.2017.00476;

curator keyword: Biological

submitter keyword: tran, tick, Salivary Glands, Blood feeding, Spotted fever,Amblyomma, RNA-Seq

Andrea Fogaca
contact affiliation	Parasitology, University of São Paulo/Institute of Biomedical Sciences, Brazil
contact email	deafog@gmail.com
lab head
Rebeca Kawahara
contact affiliation	Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia
contact email	rebecasakuma@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007852
     1. Label: PRIDE project
     2. Name: Analysis of the Salivary Gland Transcriptome of Unfed and Partially Fed Amblyomma sculptum Ticks and Descriptive Proteome of the Saliva