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PXD007845-1

PXD007845 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHEK293T LC-MS/MS .
DescriptionLeucine plays crucial roles in protein proteolytic autophagy process, but the underlying mechanisms are not fully understood. Here, we combined in vitro reconstitution with comparative proteomics technology to identify the potential regulators for leucine-mediated autophagy pathway. We established a in vitro cell-free systems to mimic the activation of autophagy by monitoring the translocation of readout protein ATG14 to the autophagosome membrane. Based on the system, we further screened the potential regulators involved in the leucine-mediated autophagy pathway using comparative proteomics strategy.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:10:23.292.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuokai Yan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-26 02:41:41ID requested
12024-10-07 10:10:23announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: HEK293T,cell-free system, leucine, iTRAQ
Contact List
Xianghua Yan
contact affiliationCollege of Animal Sciences & Technology, Huazhong Agricultural University
contact emailxhyan@mail.hzau.edu.cn
lab head
Guokai Yan
contact affiliationCollege of Animal Sciences & Technology, Huazhong Agricultural University
contact emailgkyan@webmail.hzau.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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