PXD007845-1

PXD007845 is an original dataset announced via ProteomeXchange.

Title	HEK293T LC-MS/MS .
Description	Leucine plays crucial roles in protein proteolytic autophagy process, but the underlying mechanisms are not fully understood. Here, we combined in vitro reconstitution with comparative proteomics technology to identify the potential regulators for leucine-mediated autophagy pathway. We established a in vitro cell-free systems to mimic the activation of autophagy by monitoring the translocation of readout protein ATG14 to the autophagosome membrane. Based on the system, we further screened the potential regulators involved in the leucine-mediated autophagy pathway using comparative proteomics strategy.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:10:23.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guokai Yan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-26 02:41:41	ID requested
⏵ 1	2024-10-07 10:10:23	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: HEK293T,cell-free system, leucine, iTRAQ

Xianghua Yan
contact affiliation	College of Animal Sciences & Technology, Huazhong Agricultural University
contact email	xhyan@mail.hzau.edu.cn
lab head
Guokai Yan
contact affiliation	College of Animal Sciences & Technology, Huazhong Agricultural University
contact email	gkyan@webmail.hzau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD007845

PRIDE project URI

• PRIDE
  1. PXD007845
     1. Label: PRIDE project
     2. Name: HEK293T LC-MS/MS